Healthy living cells maintain a reducing state within their cytosol. This “reducing
potential” of cells converts alamarBlue® reagent into a detectable fluorescent (or absorbent)
No. alamarBlue® reagent is a safe, non-toxic reagent to both the sample and user.
No, alamarBlue® reagent is supplied as a 10X, ready-to-use solution.
Yes. alamarBlue® reagent works on adherent and suspension mammalian cells men rings.
Yes, alamarBlue® reagent has been shown to work with bacterial2 and plant cells.4
Actually, alamarBlue® reagent is comparable to other often more expensive cytotoxicity indicators. See Figure 5 for comparison data.
alamarBlue® reagent is sensitive enough to detect less than 50 mammalian cells in a single well of a 96-well plate. See Table 3 and Figure 3 for details.
The reagent is stable for up to 12 months when stored at room temperature (~22ºC).
Yes. alamarBlue® reagent is stable to multiple freeze/thaw cycles. Be sure to heat the reagent in a 37°C water bath and mix the reagent to ensure a homogenous solution before use vintage costume jewelry.
Yes, alamarBlue® reagent is very slowly converted into a fluorescent product over time, when exposed to light, thus leading to high background values. Store the reagent, protected from light.
Incubate the cells with alamarBlue® reagent for 1–4 hours at 37°C. For more sensitive detection with low cell numbers, increase the incubation time for up to 24 hours.
Yes. However, signals from higher cell density samples may have “saturated,” which means the linearity of reagent may have reached a plateau. If this occurs, decrease the incubation time.
The absorbance of alamarBlue® reagent also changes depending on cell viability and proliferation. Therefore, simply monitor the absorbance of the reagent at 570 nm replique rolex Submariner, while using 600 nm as a reference wavelength.
No. While alamarBlue® can be used as a terminal readout of a population of cells, the reagent can also be used to continuously monitor cell viability and proliferation in real time. Since alamarBlue® reagent is non-toxic, you can incubate cells with reagent and monitor fluorescence (or absorbance) over time on the same sample.
The reagent may be breaking down due to exposure to light. Be sure to store alamarBlue® reagent in the dark and do not expose the reagent to direct light for long periods of time.
Try increasing the incubation time of cells with alamarBlue® reagent, changing the instrument’s “gain” setting, and checking the instrument filter/wavelength settings costume jewelry. Make sure to have positive controls (living cells) in the experimental design for troubleshooting.
Try decreasing the incubation time or reducing the number of cells used in the experiment