Magnetic beads are usually made of silica impregnated with iron.
What is the basis for DNA-magnetic bead binding?
Under chaotropic conditions, DNA has an affinity for silica coated beads. The DNA is released from the beads by addition of a low ionic strength buffer and heat.
What are paramagnetic beads?
Compounds that posses magnetic quality only when exposed to a magnetic force.
How large are typical magnetic beads?
Generally 3-10 microns in diameter.
Can the beads be re-used?
We do not recommend re-use of magnetic beads.
Can the beads be coated with other materials?
Yes. For example, streptavidin coated beads may be used to isolate biotin labeled proteins.
Can alkaline lysis protocols be used to release DNA?
Yes, although some modifications may be required for compatibility with MAGTRATION Technology.
What types of nucleic acids can be purified with MAGTRATION Technology?
Genomic DNA, mRNA, total RNA, plasmids
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