ข้อแตกต่างระหว่าง GeneArt Gene Synthesis Service แบบ Classic กับแบบ Instant Designer

GeneArt Instant Designer

GeneArt Strings
DNA Fragments

Value Gene
Synthesis Services

Prime Gene
Synthesis Services

Cloning delivery vectors

• Standard GeneArt delivery vector ที่มี ampicillin หรือ kanamycin resistance markers (pMX: pMA และ pMK)

• Standard GeneArt delivery vector ที่มี ampicillin หรือ kanamycin resistance markers (pMX: pMA และ pMK)

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Subcloning delivery vector

• มีบริการ Express Cloning Vectors:
- pcDNA3.1(+)
- pcDNA3.3-TOPO
- pcDNA3.4-TOPO
- pFastBac1
- pDONR221
- pET100/D-TOPO
- pET151/D-TOPO
- pRSET A
- pYes2.1V5-His TOPO

• มีบริการ Express Cloning Vectors:
- pcDNA3.1(+)
- pcDNA3.3-TOPO
- pcDNA3.4-TOPO
- pFastBac1
- pDONR221
- pET100/D-TOPO
- pET151/D-TOPO
- pRSET A
- pYes2.1V5-His TOPO

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Sequence complexity

• Non-Complex Sequences
   (GC content 20 - 80%)
• Non-secondary structure
• Non-direct/ indirect repeats complexity
• Non-long polypurine/ polypyrimidine

• Complex Sequences
   (GC content > 80%)
• Secondary structure
• Direct/ indirect repeats
• Long polypurine/ polypyrimidine

• Non-Complex Sequences
   (GC content 20 - 80%)
• Non-secondary structure
• Non-direct/ indirect repeats
• Non-long polypurine/ polypyrimidine

Sequence length

200 bp - 5,000 bp

100 bp - 12,000 bp

150 bp - 3,000 bp

Product QC method

• Next Gen Sequencing

• Sanger Sequencing
• Next Gen Sequencing

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