PiNK Plus Prestained Protein Ladder

  • Broad range: 10-175 kDa
  • Ready-to-use: supplied in a loading buffer for direct loading on gels
  • Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye
  • Sharp bands
Description
The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Contents
Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol).

Applications

 

  • Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
  • Monitoring of protein transfer onto membranes during Western blotting.
  • Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting
Storage
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
For long term storage, store at -20°C.
Recommendations for Loading
1.Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2.Mix thoroughly to ensure the solution is homogeneous.
3.Load the following volumes of the ladder on SDS-polyacrylamide gel:
– 5 μl per well for mini-gels, 2.5 μl per well for blots
– 10 μl per well for large gels, 5 μl per well for blots