D-MEM (1X), 500 ML

D-MEM (1X), 500 ML


Cat. No. : 1IVG2-11995-065
Brand : : Invitrogen

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SAVE
15%
฿ 1,100 1,300


DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With Without
• High Glucose • HEPES
• L-glutamine  
• Phenol Red  
• Sodium Pyruvate  


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical DMEM product made in our Scotland facility (41966-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

Concentration: 1X

Product Type: DMEM (Dulbecco's Modified Eagle Medium)

Sterility:  Sterile-filtered

Serum Supplementation: Standard Serum Supplementation

Shipping Condition: Room Temperature

Volume (Metric): 500 ml

Product Line: Gibco™

Form: Liquid

Glutamine: L-Glutamine

Phenol Red Indicator: Phenol Red

Shelf Life: 12 Months From Date of Manufacture

Green Features: Sustainable packaging

Quantity: 500mL

Cell Type: primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells

With Additives: High Glucose, L-Glutamine, Phenol Red, Sodium Pyruvate

Without Additives: HEPES

Glucose: High Glucose

Sodium Pyruvate Additive: Sodium Pyruvate

For Use With (Application): Mammalian Cell Culture

Cell Line: HeLa, 293, Cos-7, and PC-12

Classification: Animal Origin-Free

Solution Type: DMEM (Dulbecco's Modified Eagle Medium)

HEPES Buffer: No HEPES

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