qTOWER 3.0 STANDARD REAL-TIME PCR, BODY ONLY (NO GRADIENT)
qTOWER3 - Real-Time PCR Thermal Cycler
- Hands-on: Stand-alone operation via integrated tablet control (10“) and/or enhanced PC control
- Transparent: No costs for license or updates (license-free)
- Universal: Covering a broad range of analysis tools from a simple overview of Ct values to ddCT method and multi plate analysis
- Multilingual: Available in multiple different languages
- Convenient: 12 available Color, FRET and Protein Modules can be installed or exchanged within a few minutes
- Future-proof: Adaptable to new applications at any time by adding filter modules
- Robust: Patented high performance optical system with an outstanding long-term warranty of 10 years!
- Efficient: Short scan times of 6 seconds for the entire plate, irrespective of the number of dyes detected
- Innovative: Novel light source with 4 robust LEDs (RGBW) – no preheating time, long life-time
- Brillant: Ideal illumination and detection of all 96 wells individually without edge effect due to fiber optic shuttle
- Fast: Heating rates of 8 °C/sec and cooling rates of 6 °C/sec
- Unrivaled: Ideal temperature homogeneity and unmatched control accuracy (± 0.1 °C)
- Precise: The temperature difference from lane to lane is the same; simple programming of even gradient temperatures is possible with our unique Linear Gradient Tool (LGT)
| Technical Data | |||
| Thermal block | |||
| Sample block | Silver sample block with gold-coating | ||
| Block capacity | 96 Well á 0.2 ml for 96 x 0.2 ml | ||
| Sample size | 10 – 80 μl | ||
| Heating | Up to 8 °C/sec (max.) | ||
| Cooling | Up to 6 °C/sec (max.) | ||
| Adjustable temperature range | 4 °C to 99 °C | ||
| Temperature uniformity | 55 °C ± 0.15 °C after 15 sec | ||
| Temperature control accuracy | ± 0.1 °C | ||
| Gradient | Linear Gradient Tool | ||
| Max./Min. Gradient | 40 °C / 0.1 °C | ||
| Heated lid | |||
| Lid temperature | 30 °C to 110 °C | ||
| Contact pressure | 30 kg, automated | ||
| qPCR application | |||
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA | ||
| Dynamic range | 10 orders of magnitude | ||
| Optics | |||
| Measuring principle | Fiber optic shuttle system with 8-fold scanner and color modules for excitation and emission filters |
||
| Light source | 4 longlife, high-power LED’s (RGBW) | ||
| Detector | High sensitive PMT (Photo Multiplier Tube) |
||
| Color modules | 12 Color-, FRET- and Protein Modules 6 positions inside device |
||
| Read out time | 6 sec for 96 wells independent of the number of dyes | ||
| Control | |||
| Control | PC or stand-alone (optional) | ||
| Display | Only qTOWER³ touch: 10“ tablet, colored, touch, WIN 8.1 | ||
| Time inc/dec | ±1 to 240 sec/cycle | ||
| Temperature inc/dec | ±0.1 to 20 °C/cycle | ||
| Additional technical data | |||
| Weight | Approx. 30 kg | ||
| Dimension (W x H x L) |
275 mm x 585 mm x 275 mm | ||
| Interface | PC connection: USB, RS232, Tablet: USB for data transfer, barcode reader |
||
| Noise emission | 45 db | ||
| Warranty | 2 years warranty on device system 10 years long-term warranty of high performance optics |
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| Parameters: color modules | |||
| Name | Excitation | Emission | Dyes* (examples) |
| Color module 1 | 470 nm | 520 nm | FAMTM, Sybr®Green, Alexa488® |
| Color module 2 | 515 nm | 545 nm | JOETM, HEXTM, VIC®, YakimaYellowTM |
| Color module 3 | 535 nm | 580 nm | TAMRATM, DFOTM, Alexa546®, NEDTM |
| Color module 4 | 565 nm | 605 nm | ROXTM, TexasRed®, Cy3.5® |
| Color module 5 | 630 nm | 670 nm | Cy5®, Alexa633®, Quasar670TM |
| Color module 6 | 660 nm | 705 nm | Cy5.5®, LightCycler Red® |
| FRET module 1 | 470 nm | 580 nm | FAMTM (donor) / TAMRATM(acceptor) |
| FRET module 2 | 470 nm | 670 nm | FAMTM (donor) / Cy5®(acceptor) |
| FRET module 3 | 470 nm | 705 nm | FAMTM (donor) / Cy5.5®(acceptor) |
| FRET module 4 | 515 nm | 670 nm | JOETM (donor) / Cy5®(acceptor) |
| FRET module 5 | 470 nm | 605 nm | FAMTM (Donor)/ROXTM (acceptor) |
| Color modul Protein 1 | 490 nm | 580 nm | SYPRO® Orange |
| * Yakima Yellow is registered trademark of Epoch Biosciences, Inc. Cy is a trademark of GE Healthcare. FAM, HEX, JOE, VIC, TAMRA, NED and ROX are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. SYBR, Alexa Fluor, SYPRO and Texas Red are registered trademarks of Molecular Probes, Inc. TaqMan and LightCycler are registered trademarks of Roche Group, Inc. Quasar Dyes are trademarks of Biosearch Technologies Inc. DFO™ is a trademark of Eurogentec S.A. Windows and Excel are trademarks of Microsoft Corporation. | |||
| Order number | Description |
| 844-00520-0 | Color module 1 - FAMTM, Sybr®Green, Alexa488® |
| 844-00521-0 | Color module 2 - JOETM, HEXTM, VIC®, YakimaYellowTM |
| 844-00522-0 | Color module 3 - TAMRATM, DFOTM, Alexa546®, NEDTM |
| 844-00523-0 | Color module 4 - ROXTM, TexasRed®, Cy3,5® |
| 844-00524-0 | Color module 5 Cy5®, Alexa633®, Quasar670TM |
| 844-00525- 0 | Color module 6 - Cy5.5®, LightCycler Red® |
Product brochure qTOWER³ Product Family Click Here
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