The Invitrogen SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits.

Specifications

Concentration: 2 X

Detection Method: Gel Electrophoresis

Fidelity (vs. Taq): 2 X

Format: Tube

GC-Rich PCR Performance: High

Hot Start: Built-In Hot Start

No. of Reactions: 100

PCR Method: 1-Step RT-PCR

Polymerase: Platinum™ SuperFi™ DNA Polymerase

Reaction Speed: Fast

Sample Type: RNA

Size (Final Product): 14 kb or less

Volume: 0.2 mL

No. of Tests: 100 Reactions

Product Line: SuperScript™

Product Type: One-Step RT-PCR System

Reverse Transcriptase: SuperScript™ IV

Shipping Condition: Dry Ice

Sufficient For: 100 Reactions