Vivacon 2-PCR Grade
100 µl - 2 ml
Vivacon® 2 are centrifugal units for fast DNA concentration and buffer exchange of 2 ml samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® ultrafiltration membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step.
Vivacon® 2-PCR Grade units are treated with ethylene oxide (ETO) in a validated process to deactivate all traces of DNA that might interfere with subsequent amplification procedures.
ivacon® 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 2 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.
High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62.
- Concentration of up to 2 ml genomic DNA or peptide samples
- Low binding material
- Reverse spin possibility
- MWCO printed on device
- Complete and highly reproducible concentrate recovery
- Selected cut offs available sterile and DNA free* for e.g. forensic applications
- * qPCR assay over 55 cycles with a verified limit of detection < 2 pg
Performance Characteristics for DNA
Start volume 2 ml, sample concentration 50 ng/ml.
| Sample size (bp) | Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) | |
|---|---|---|---|---|
| 2,000 MWCO | 10 | 120 min. | 92 % | 7,500 |
| 10,000 MWCO | 30 | 60 min. | 94 % | 5,000 |
| 30,000 MWCO | 50 | 60 min. | 95 % | 2,500 |
| 50,000 MWCO | 300 | 45 min. | 96 % | 2,500 |
| 100,000 MWCO | 600 | 30 min. | 93 % | 2,500 |
| 125,000 MWCO | 650 | 30 min. | 88 % | 2,500 |
| 125,000 MWCO | 900 | 30 min. | 89 % | 2,500 |
Performance Characteristics for Proteins
Start volume 2 ml, sample and concentration of proteins as specified in table.
| Sample | Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) | |
|---|---|---|---|---|
| 2,000 MWCO | 0.25 mg/ml cytochrome c |
120 min. | 95 % | 7,500 |
| 10,000 MWCO | 0.25 mg/ml cytochrome c |
90 min. | 96 % | 5,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 40 min. | 96 % | 5,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 30 min. | 94 % | 5,000 |
| 100,000 MWCO | 1.0 mg/ml bovine IgG |
30 min. | 92 % | 5,000 |
| 125,000 MWCO | 1.0 mg/ml bovine IgG |
27 min. | 81 % | 5,000 |
Start volume 2 ml, sample and concentration of proteins as specified in table.
| Sample | Time to concentrate up to 30x [min.] at 20°C |
Concentrate recovery % |
g-force (xg) | |
|---|---|---|---|---|
| 2,000 MWCO | 0.25 mg/ml cytochrome c |
120 min. | 95 % | 7,500 |
| 10,000 MWCO | 0.25 mg/ml cytochrome c |
90 min. | 96 % | 5,000 |
| 30,000 MWCO | 1.0 mg/ml BSA | 40 min. | 96 % | 5,000 |
| 50,000 MWCO | 1.0 mg/ml BSA | 30 min. | 94 % | 5,000 |
| 100,000 MWCO | 1.0 mg/ml bovine IgG |
30 min. | 92 % | 5,000 |
| 125,000 MWCO | 1.0 mg/ml bovine IgG |
27 min. | 81 % | 5,000 |
| Product properties | |
| MWCO | 30,000 Daltons |
| Volume Range | 0.1 – 2 mL |
| Primary Product Type | Ultrafiltration Devices |
| PCR Grade | Yes |
| Membrane material | Hydrosart® |
| Membrane | Hydrosart<sup>®</sup> |
| Technical attributes | |
| Concentrator Cap | Polypropylene (PP) |
| Concentrator Body/ Sleeve | Polycarbonate (PC) |
| Dead stop volume | 55 μl |
| Certification | None |
| Filtrate Vessel | Polypropylene (PP) |
| Application | Protein Concentration` DNA Concentration |
| Area of application | Buffer Exchange` Forensic Sample Prep` Protein/DNA Concentration |
| Market | Forensic |
| Device type | Centrifugal Ultrafiltration Devices |
| Sample TypeDNA | DNA |
Ultrafiltration Family Click Here
Laboratory Filtration Products Click Here
Laboratory Filtration Products (US and Canada) Click Here
Vivacon® 500 and Vivacon® 2 — For safe DNA and protein concentration and rebuffering from 500 μl to 2 ml starting volumes. Click Here
Vivacon® 2 — For in vitro use only Click Here
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