TAQMAN GENE EXPRESSION ASSAY, VIC, 360 RXN
Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.
Features include:
- Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
- Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
- Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
- Accurate—identify small fold-changes with high accuracy of quantitation
- Extensive content—over 1.8 million predesigned assays available for over 25 different species
- Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
- Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
Contents & Storage
1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).
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Store at -15 to -25°C.
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