SENSICOLUMN IAC ERGOT ALKALOIDS (3 ML X 50 COLUMNS)

SENSICOLUMN IAC ERGOT ALKALOIDS (3 ML X 50 COLUMNS)


Cat. No. : 5GSD-EFLS3275
Brand : : GoldStandard Diagnostics




This instruction of priority ergot alkaloid determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with HPLC. Accepted laboratory extraction and IAC treatment methods could essentially be maintained. Full performance of the IAC column is given if pronounced criteria of organic solvent tolerance, elution process and working range of SENSIColumn Ergot Alkaloids 3ml column is followed.

Many pretreatment methods of ergot alkaloids determination in food and feed show low sensitivity because of interfering substances if problematic matrices are applied. This method of content determination of ergot alkaloids combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate analytes and additional step of purification by HPLC column. Please notice that this instruction focuses on the handling with the IAC column. For the commodity extraction step a literature method is given. The given apparatus (e.g. HPLC system) might serve as an example among other possibilities.

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