DNANIMAL IDENT PORK IPC KIT, 96 REACTIONS
The DNAnimal Ident Pork IPC real-time PCR detection kit provides materials for the qualitative detection of porcine DNA. The kit detects the target sequence in food and feed sample types from which DNA in sufficient quality and quantity can be extracted. Note: A differentiation between domestic pig and wild boar (sus scrofa domestica and sus scrofa scrofa) is not possible with this kit. Both will be detected equally.
The kit described in this instruction for use was designed for real-time PCR instruments, that have at least the following detection channels: FAM™ and HEX™/VIC®. When using a real-time cycler which requires a normalization with ROX as passive reference dye, the additional detection channel TAMRA is required. The performance of the kit has been validated on various instruments, details can be found in the kit-specific validation report, which is available upon request. For all real-time instruments in-scope of the official validation, an Excel-based evaluation sheet is also available upon request.
The procedure of the current kit follows the basic principles of the real-time PCR amplification method. DNA segments are amplified with specific primers. PCR products are measured during each cycle (real-time) with target-specific oligonucleotide probes labelled with fluorescent reporter dyes, which permits detection only after hybridization of the probe with its complementary sequence of the amplified PCR product. An internal positive control (IPC) contained in the Master Mix is amplified in parallel and indicates inhibition, if it occurs, thus avoiding false-negative/inhibited results for every individual sample.
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