DNANIMAL IDENT PIG HS IPC (LR) HIGH SENSITIVITY, 96 REACTIONS
The DNAnimal Ident Pig HS IPC (LR) real-time PCR detection kit provides materials for the qualitative detection of pig DNA. The kit detects the target sequence in food and feed sample types from which DNA in sufficient quality and quantity can be extracted. The kit assay targets a mitochondrial gene of pig. Depending on the tissue type cells contain several thousand mitochondrial DNA copies which lead to an extremely high sensitivity of the assay. Due to this very high sensitivity of the assay, the risk of contamination is very high. Therefore, this assay is only recommended for low DNA samples (e.g. gelatine) or in cases where very high sensitivity is required. In labs where pork samples are analysed on a regular basis and where the risk of contamination is very high, we recommend our DNAnimal Ident Pork IPC kit. With this kit, it is not possible to differentiate between domestic pig and wild boar (sus scrofa domestica and sus scrofa scrofa). Both will be detected equally.
The kit described in this instruction for use was designed for real-time PCR instruments, that have at least the following detection channels: FAM™ and HEX™/VIC®. When using a real-time cycler which requires a normalization with ROX as passive reference dye, the additional detection channel TAMRA is required. The performance of the kit has been validated on various instruments, details can be found in the kit-specific validation report, which is available upon request. For all real-time instruments in-scope of the official validation, an Excel-based evaluation sheet is also available upon request.
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