SUPERSCRIPT VILO cDNA SYNTHESIS KIT, 50 RXN
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.
Specifications
Concentration: 10 X
Detection Method: Primer-Probe
Fidelity (vs. Taq): 10 X
Format: Tube
GC-Rich PCR Performance: High
No. of Reactions: 50 Reactions
PCR Method: qPCR, RT-PCR
Reaction Format: SuperMix or Master Mix
Sample Type: RNA
Size (Final Product): 10 kb or less
Volume: 20 μL
For Use With (Application): Real-Time Quantitative PCR (qPCR)
No. of Tests: 50 Reactions
Optimal Reaction Temperature: 42° C
Primer: Random Primers
Product Line: SuperScript™, VILO™
Product Type: cDNA Synthesis Kit
Quantity: 50 reactions
Reverse Transcriptase: SuperScript™ III
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
Sufficient For: 50 Reactions
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