SUPERSCRIPT III 1st STRAND SYNTHESIS SYS.
The SuperScript® III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.
Specifications
Format: Tube
GC-Rich PCR Performance: High
No. of Reactions: 50 Reactions
PCR Method: RT-PCR
Reaction Format: Separate Components
Volume: 20 μL
For Use With (Application): Microarray Analysis, Reverse Transcriptase PCR (RT-PCR)
No. of Tests: 50 Reactions
Optimal Reaction Temperature: 50° C
Product Line: SuperScript™
Product Type: First-Strand Synthesis System
Reverse Transcriptase: SuperScript™ III
Shipping Condition: Dry Ice
Sufficient For: 50 Reactions
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