SS III ONE-STEP W/PLATINUM TAQ, 100 RXN
The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers, and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript® III Reverse Transcriptase and Platinum® Taq DNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb. The amount of starting material can range from 0.01 pg to 1 µg of total RNA. Key advantages of this kit:
• Sensitive—routine detection down to 0.01 pg total RNA (see figure)
• Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
• Specificity—uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers (see figure)
• Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
Specifications
Detection Method: Gel Electrophoresis
Format: Tube
GC-Rich PCR Performance: High
Hot Start: Built-In Hot Start
No. of Reactions: 100 Reactions
PCR Method: 1-Step RT-PCR
Polymerase: Platinum™ Taq DNA Polymerase
Sample Type: RNA
Size (Final Product): 4.5 kb or less
Volume: 200 μL
For Use With (Application): RT-PCR
Includes: 200μL SuperScript III RT/Platinum Taq Mix; 3 × 1mL 2X Reaction Mix (containing 0.4mM of each dNTP, 3.2mM MgSO4); 500μL 5mM Magnesium Sulfate
No. of Tests: 100 Reactions
Primer Probe Compatibility: Unlabeled Oligos
Product Line: Platinum™, SuperScript™
Product Type: One-Step RT-PCR System
Quantity: 100 reactions
Reverse Transcriptase: SuperScript™ III
Shipping Condition: Dry Ice
Sufficient For: 100 Reactions
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