SENSISPEC INGEZIM HYDROLYZED GLUTEN 48 WELLS
INgezim® GLUTEN HIDROLIZADO is a direct enzyme immunoassay. It can identify and quantify hydrolyzed prolamins (gliadina, hordein and secalins). Prolamins extracted from the sample are chemically fixed to the polystyrene plate wells. After a washing step, the peroxidaseconjugated R5 Mab is added, which will bind to the fixed prolamins. After the addition of the substrate, a colorimetric reaction occurs. This reaction is directly proportional to the amount of gluten in the well. With INgezim® GLUTEN HIDROLIZADO is possible to quantify the amount of hydrolyzed gluten of a sample with a detection limit of 0,25 ppm.
|
Test principle |
Direct enzyme immunoassay |
|
Format |
Microtiter plate with 48 wells |
|
Incubation Time |
75 min (30/30/15 min) |
|
Standards |
400 ppm (Gluten). To prepare the following standards: 2; 1; 0.5; 0.25; 0.125 ppm (Gluten) |
|
Detection |
450 nm |
|
Detection limit (LOD) |
0.25 ppm |
|
Quantitation Range |
0.25-250 ppm |
|
Cross-reactivities |
No |
|
Shelf Life (months) |
12 |
|
Storage |
4°C |
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