SENSISPEC ELISA CASEIN, 48 WELLS
Bovine milk belongs to the most important allergenic food ingredients especially for children. Already very low amounts of bovine milk can cause allergic reactions, which may lead to anaphylactic shock in severe cases. In particular the presence of hidden milk proteins such as in sausage, cookies, convenience food or beverages represent a critical problem for milk allergic persons.
Approximately 80% of bovine milk proteins are caseins which are composed of α-, β- and κ-caseins. So these heat-stable allergens represent the main fraction of bovine milk proteins.
The SENSISpec Casein ELISA represents a highly sensitive detection system and is particularly capable of the identification and quantification of bovine casein residues in cookies, bread crumbs, sausage, orange juice, wine, soy products and chocolate.
Test principle
‣ Sandwich enzyme immunoassay
Format
‣ Microtiter plate
Incubation Time
‣ 60 min (20/20/20 min)
Standards
‣ 0; 0.2; 0.6; 2; 6 ppm
Detection
‣ 450 nm (HRP + TMB)
Detection limit (LOD)
‣ 0.04 ppm
Quantitation Range
‣ 0.2 - 6 ppm
Cross-reactivities
‣ Sheep’s (ewe’s) milk 1.2% Goat’s milk 1.1%
Shelf Life (months)
‣ 13
Storage
‣ 2–8°C
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