SENSISPEC ELISA BETA-LACTOGLOBULIN, 48 WELLS
Bovine milk belongs to the most important allergenic food ingredients especially for children. Already very low amounts of bovine milk can cause allergic reactions, which may lead to anaphylactic shock in severe cases. In particular the presence of hidden milk proteins such as in sausage, cookies, convenience food or beverages represent a critical problem for milk allergic persons.
Approximately 20% of bovine milk proteins are whey proteins. The main fraction of whey proteins consists of the heat-stable allergen ß-lactoglobulin.
The SENSISpec ß-Lactoglobulin ELISA represents a highly sensitive detection system and is particularly capable of the identification and quantification of bovine milk residues in cookies, cereals, sausage, orange juice, wine, soy products and chocolate.
Test principle
‣ Sandwich enzyme immunoassay
Format
‣ Microtiter plate
Incubation Time
‣ 60 min (20/20/20 min)
Standards
‣ 0; 10, 40; 100; 400 ppb
Detection
‣ 450 nm (HRP + TMB)
Detection limit (LOD)
‣ 1.5 ppb
Quantitation Range
‣ 10 - 400 ppb
Cross-reactivities
‣ Ewe’s (sheep’s) milk < 0.2% Goat’s milk < 0.002% Casein < 0.02%
Shelf Life (months)
‣ 13
Storage
‣ 2–8°C









