The DNAExtractor Honey kit is designed for the extraction of high-quality and purity DNA from honey and pollen. The extracted DNA can be used for all kinds of subsequent analytical methods, e.g. real-time and conventional

PCR testing. Please note that the kit does not contain the solvents necessary for extraction due to freight security reasons. 

The DNA isolation process of the DNAExtractor Honey kit is based on an organic extraction using cetyl trimethylammonium bromide (CTAB) lysis, chloroform extraction and DNA precipitation. After harvesting and disruption of the pollen in the sample, lysis is performed by incubation with lysis buffer containing CTAB and Proteinase K which possess the property to release the DNA. The extraction is then carried out using chloroform where the DNA remains in the aqueous phase whereas other components of the sample go into organic phase. In the subsequent step DNA is precipitated with isopropanol. The following washing step with ethanol removes remaining reagents and salts that could inhibit the subsequent applications. Afterwards, the DNA is solved in low salt buffer or water to be ready-to-use for further analysis or amplification. Since the composition and biochemistry of the different sample types is very heterogeneous, the extraction of DNA can be a challenging procedure. Depending on the sample type and the subsequent application, further cleaning of the DNA can be required, e.g. with DNA Cleaning Columns (cat. no. 5224700310).