0.5 - 5 l

• Plug and play laboratory cross flow cassette
• Re-usable
• Optimal for cell culture supernatant and virus concentration
• High flow rate and recovery

• Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 5 l samples. The membrane surface per device is 200 cm². One unit comes with all necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up the concentration, two cassettes can be run simultaneously.

  Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities. This economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 200 cm2 module typically concentrates 1 l by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 modules can be used in parallel for concentration of 5 l in under 75 minutes. Near total recovery of the concentrate is achieved with a single final rinse. Vivaflow® 200 can be easily cleaned and re-used several times. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package for using a single cassette. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.

  Equipment required

  The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

  Equipment required

  The standard Vivaflow® 200 package comes with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).

  Running 2 Vivaflow® 200 cassettes in parallel

  For speeding up the concentration, two Vivaflow® 200 cassettes can easily be connected using a Y connector which is preassembeled with the correct tubing sizes and fittings (size 16 tubing for connecting the two Vivaflow® 200 units, size 15 tubing for the pump). Please note that you need a size 15 pump head for running 2 Vivaflows® in parallel.

  • High flow rate and recovery
  • Clear housing for process monitoring
  • Ready to use single cassettes
  • Easy set up of 2 cassettes for use in parallel
  • Re-usable product

  Performance Characteristics

  	Time to concentrate up to 20x (min.) at 3 bar inlet pressure, 20°C
  	Start volume	Average flux	Recovery %
  	1 litre	ml/min	Direct	25 ml rinse
  Lysozyme 0.25 mg/ml (14,000 MW)
  2,000 MWCO Hydrosart	160	6	97 %	>99 %
  3,000 MWCO PES	180	5	97 %	>99 %
  BSA 1.0 mg/ml (66,000 MW)
  5,000 MWCO PES	29	33	98 %	>99 %
  5,000 MWCO Hydrosart	70	14	98 %	>99 %
  10,000 MWCO PES	23	41	96 %	>99 %
  10,000 MWCO Hydrosart	35	27	98 %	>99 %
  30,000 MWCO PES	25	38	96 %	99 %
  30,000 MWCO Hydrosart	20	48	96 %	>99 %
  50,000 MWCO PES	22	43	96 %	98 %
  γ Globulins 1.0 mg/ml (average 160,000 MW)
  100,000 MWCO PES	54	18	96 %	99 %
  100,000 MWCO RC	45	21	96 %	99 %
  Yeast 1.0 mg/ml (S. Cerevisiae)
  0.2 μm PES	11	86	92 %	98 %
  Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES
  BSA 0.001 mg/ml	18	52	90 %	98 %
  BSA 0.01 mg/ml	20	47	92 %	98 %
  BSA 0.1 mg/ml	21	45	94 %	99 %
  Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES
  BSA 1.0 mg/ml (66,000 MW)	67	70	97 %	>99 %

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