VIVASPIN 20, 3K, 12/PKG

VIVASPIN 20, 3K, 12/PKG


Cat. No. : 2SRVS-VS2091
Brand : : Sartorius Lab Instruments


5 - 20 ml
Ultrafiltration unit with unique twin membrane for sample concentration with high process flexibility: centrifugation or centrifugation of pressurized units, as well as pressure filtration in stationary benchtop mode.

Vivaspin® 20 centrifugal concentrators offer increased volume flexibility and performance to the Vivaspin® potfolio. Vivaspin® 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin® 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows monitoring the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin® 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is can be placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin® 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. 'Pressure-fugation' is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.

  • High flux PES membrane
  • High retentate recovery > 90 %
  • Vertical twin membrane for ultra-fast concentration
  • Universal rotor compatibility
  • Safe sample concentration due to dead-stop volume
  • Convenient concentrate recovery by pipetting
  • Volume graduation for easy reading/monitoring
  • 100-fold concentration with twin vertical membranes
  • Additional option for pressurized operation

Rotor Compatibility

Please note: Vivaspin® 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin® Turbo 15, which can be used in either round bottom or conical centrifuge tubes

Performance Characteristics

 

  Time to concentrate up to 30x [min.] at 20°C
and solute recovery %
Mode Centrifuge Centrifuge Bench top Press-fuge
Rotor Swing bucket 25° Fixed angle Pressure Swing bucket
Sart volume 20 ml 14 ml 10 ml 10 ml
  Min./Rec. Min./Rec. Min./Rec. Min./Rec.
Cytochrome c 0.25 mg/ml (12,400 MW)
3,000 MWCO PES 110 / 97 % 180 / 96 % 60 / 96 % - / -
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 23 / 99 % 29 / 99 % 50 / 98 % 14 / 98 %
10,000 MWCO PES 16 / 98 % 17 / 98 % 32 / 97 % 8 / 97 %
30,000 MWCO PES 13 / 98 % 15 / 98 % 32 / 97 % 8 / 97 %
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES 27 / 97 % 20 / 95 % 46 / 94 % 13 / 97 %
50,000 MWCO PES 27 / 96 % 22 / 95 % 46 / 93 % 13 / 96 %
100,000 MWCO PES 25 / 91 % 20 / 90 % 42 / 88 % 12 / 94 %
Latex beads 0.004% in DMEM 10% FCS (0.055 μm)
300,000 MWCO PES 20 / 99 % 35 / 99 % 10 / 99 % - / -
Latex beads 0.004% in DMEM 10% FCS (0.24 μm)        
1,000,000 MWCO PES 4 / 99 % 12 / 99 % 4 / 99 % - / -
Yeast 1.0 mg/ml (S. Cerevisiae)
0.2 μm PES 15 / 95 % 5 / 95 % 20 / 95 % 2 / 95 %

 

Product properties  
MWCO 3,000 Daltons
Width 30 mm
Volume Range 5 – 20 mL
Primary Product Type Ultrafiltration Devices
Membrane material Polyethersulfone (PES)
Membrane Polyethersulfone (PES)
Technical attributes  
Electrical / battery item false
Pressure Head / Pressure-fuge Head Acetal / Aluminium
Concentrator CapPolypropylene (PP) Polypropylene (PP)
Concentrator Body/ Sleeve Polycarbonate (PC)
Overall length 124.5 mm*, 117 mm
Hold-up volume < 20 µL
Active Membrane Area 5.8 cm²
Swing Bucket Rotor 20 mL
Dead stop volume 50 µL
Fixed Angle Rotor 14 mL
With Pressure Head 15 mL
Certification None
Filtrate Vessel Polycarbonate (PC)
Application Protein Concentration
Area of application Buffer Exchange` Protein/DNA Concentration
Market BioPharma` Research
Device type Centrifugal Ultrafiltration Devices` Pressure Driven Ultrafiltration Devices
Sample Type Protein` Peptide` Nanoparticles

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