VIVASPIN 2, 3K, 25/PKG
0.4 - 2 ml
Three membrane alternatives: PES, Hydrosart® regenerated Cellulose, Cellulosetriacetat. Best recovery of the concentrate by reverse-spinning.
Vivaspin® concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin® 2 can effectively be used in swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high speed concentrations, even with particle-laden solutions. Vivaspin® 2 combines the speed of the classic Vivaspin® products with low internal surface and membrane area in order to achieve superior recoveries from very dilute solutions. Available with a choice of PES, Cellulose Triacetate or Hydrosart® membranes, Vivaspin® 2 offers the highest flexibility for process optimization. Another unique feature of Vivaspin® 2 is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator or alternatively reverse-spinning the concentrate into the recovery cap. Both methods result in near total concentrate recovery.
- Choice of three different membranes
- High retentate recovery > 90 %
- Vertical membrane design for ultra-fast concentration
- Universal rotor compatibility
- Safe sample concentration due to dead-stop volume
- Convenient concentrate recovery by pipetting
- Optional re-spin for concentrate recovery
- Volume graduation for easy reading/monitoring
Performance Characteristics
| Time to concentrate up to 30x [min.] at 20°C | Concentrate recovery % | |
|---|---|---|
| Start volume | 500 μl | 500 μl |
| Aprotinin 0.25 mg/ml (6,500 MW) | ||
| 3,000 MWCO PES | 30 | 96 % |
| BSA 1.0 mg/ml (66,000 MW) | ||
| 5,000 MWCO PES | 15 | 96 % |
| 10,000 MWCO PES | 5 | 96 % |
| 30,000 MWCO PES | 5 | 95 % |
| IgG 0.25 mg/ml (160,000 MW) | ||
| 30,000 MWCO PES | 10 | 96 % |
| 50,000 MWCO PES | 10 | 96 % |
| 100,000 MWCO PES | 10 | 96 % |
| Product properties | |
| MWCO | 3,000 Daltons |
| Width | 18 mm |
| Volume Range | 0.4 – 2 mL |
| Primary Product Type | Ultrafiltration Devices |
| Membrane material | Polyethersulfone (PES) |
| Membrane | Polyethersulfone (PES) |
| Technical attributes | |
| Electrical / battery item | false |
| Concentrator Cap | Polycarbonate (PC) |
| Concentrator Body/ Sleeve | Polycarbonate (PC) |
| Overall length | 127 mm |
| Hold-up volume | < 10 µL |
| Active Membrane Area | 0.7 cm² |
| Swing Bucket Rotor | 3 mL |
| Dead stop volume | 8 µL |
| Fixed Angle Rotor | 2 mL |
| Certification | None |
| Filtrate Vessel | Polycarbonate (PC) |
| Application | Protein Concentration |
| Area of application | Buffer Exchange` Protein/DNA Concentration |
| Market | BioPharma` Research |
| Device type | Centrifugal Ultrafiltration Devices |
| Sample Type | Protein` Peptide` Nanoparticles |
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Vivaspin® 500 μl and 2 ml Click Here
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