Vivacell 70
10 - 70 ml
Ultrafiltration unit for sample concentration with highest process flexibility: Centrifugation or centrifugation of pressurized units, as well as pressure filtration in stationary benchtop mode or with gentle shaking.
ivacell® 70 is a disposable ultrafiltration unit for the concentration of samples between 10 ml and 70 ml and can be used either in a centrifuge or for pressure filtration. Vivacell® 70 thus combines the ease of use of centrifugal devices with the flexibility and control provided by pressurized ultrafiltration cells. Vivacell® 70 is easy to assemble, requires no tubing connections or stirring mechanisms and can be adapted to equipment availability or to specific user preferences. For convenience, the units are simply spun in a large capacity centrifuge (carriers accepting 250ml bottles). For even faster concentration, particularly with difficult samples, the device can be pressurized with air or inert gas before centrifuging. For better process control or for handling of single samples, gas pressure can be combined with gentle orbital shaking. For highest simplicity of use with minimum equipment requirements, the device is pressurized and then left standing on a bench top or in a refrigerator. The concentrator features a vertical membrane that minimizes fouling, while the built-in deadstop of 150 µl will prevent concentration to dryness.
- Easy to handle
- High flux PES membrane
- Vertical membrane design for ultra fast concentration
- High retentate recovery > 90 %
- Total process flexibility
- Universal rotor compatibility
Concentrator bodies with ploycarbonate filtrate bottles
Performance Characteristics
| Time to concentrate 30x min at 20°C | |||||
|---|---|---|---|---|---|
| 50 ml start volume | In centrifuge 1,000 g |
As pressure cell 4 bar (60 psi) pressure |
Solute recovery % | ||
| No Pressure | 3 bar pressure | No agitation | Orbital shake | ||
| BSA 1.0 mg/ml (66,000 MW) | |||||
| 5,000 MWCO PES | 37 | 18 | 50 | 25 | 96 % |
| 10,000 MWCO PES | 25 | 15 | 45 | 20 | 96 % |
| 30,000 MWCO PES | 22 | 13 | 45 | 20 | 93 % |
| IgG 0.25 mg/ml (160,000 MW) | |||||
| 50,000 MWCO PES | 25 | 15 | 85 | 20 | 94 % |
| 100,000 MWCO PES | 15 | 11 | 90 | 18 | 90 % |
| Product properties | |
| MWCO | 5,000 Daltons |
| Width | 62 mm |
| Volume Range | 10 – 70 mL |
| Max. operating pressure | 5 bar |
| Primary Product Type | Ultrafiltration Devices |
| Membrane material | Polyethersulfone (PES) |
| Membrane | Polyethersulfone (PES) |
| Technical attributes | |
| Electrical / battery item | false |
| Pressure Head / Pressure-fuge Head | Acetal |
| Rotor Cavity | To fit 250 mL (62 mm) centrifuge bottles |
| Concentrator Body/ Sleeve | Polycarbonate (PC) |
| Maximum Speed | 1,200 g |
| Minimum effective speed | 500 g |
| Maximum Pressure | 5 bar |
| Overall length | 119 mm |
| Hold-up volume | < 200 µL |
| Swing Bucket Rotor | 70 mL |
| Total length with Pressure-fuge head | 185 mm |
| Dead stop volume | 150 µL |
| Recommended Speed | 1,000 g |
| Fixed Angle Rotor | 50 mL |
| With Pressure Head | 70 mL |
| Minimum Rotor Ange | l25° |
| With Pressure-fuge Head | 50 mL |
| CertificationNone | None |
| Filtrate Vessel | Polycarbonate (PC) |
| Filtrate Container | Polycarbonate (PC) |
| Application | Protein Concentration |
| Area of application | Buffer Exchange` Protein/DNA Concentration |
| Market | Research |
| Device type | Centrifugal Ultrafiltration Devices` Pressure Driven Ultrafiltration Devices |
| Sample Type | Protein` Peptide` Nanoparticles |
| Rotor Type | Swing-out rotors` Angle rotors |
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